Études in vivo des propriétés antidiabétiques

Artemisia afra Jacq.

Cet article regroupes les publications concernant les études in vivo des propriétés antidiabétiques d’Artemisia afra Jacq

4

Études in vivo des propriétés antidiabétiques

Artemisia afra Jacq.

1989

Muriuki, Gichuru, Addae-Mensah, I, Guantai, Anastasia N.
A pharmacological investigation of the hypoglycemic activity of Artemisia afra Jacq.
Planta Medica 1989 ; 55(1) : 94

A pharmacological investigation of the hypoglycemic activity of Artemisia afra Jacq.
Planta Medica

2011

Anthony Jide Afolayan & Taofik Olatunde Sunmonu
Artemisia afra Jacq. ameliorates oxidative stress in the pancreas of streptozotocin-induced diabetic Wistar rats.
Bioscience, Biotechnology, and Biochemistry, Volume 75, 2011 - Issue 11

Artemisia afra Jacq. ameliorates oxidative stress in the pancreas of streptozotocin-induced diabetic Wistar rats

Abstract

Diabetes is characterized by hyperglycemia resulting from defects in pancreatic insulin secretion and/or impaired target cell responsiveness to insulin, and Artemisia afra Jacq. is widely used in South Africa to treat the disease, but the mechanism of action is yet to be elucidated. This study explored the effect of oral administration of aqueous leaf extract of A. afra on the pancreas of streptozotocin-induced diabetic rats. We found that the extract significantly reduced blood glucose levels, accompanied by an increase in the serum insulin concentration. Moreover, the antioxidant enzymic activities of glutathione peroxidase, glutathione reductase, and superoxide dismutase also improved significantly after treatment with the extract. Increased pancreatic lipid peroxidation in the diabetic rats was also normalized by the extract. This study indicates that Artemisia afra possesses hypoglycemic and antioxidant activities. Our findings suggest that the herb might exert its anti-diabetic activity by regenerating pancreatic beta cells, thereby stimulating the release of insulin.
Key words : Artemisia afra, antioxidant enzyme, diabetes, lipid peroxydation, pancreas.

2012

Afolayan AJ., Sunmonu TO.
Protective role of Artemisia afra aqueous extract on tissue antioxidant defense systems in streptozotocin-induced diabetic rats
African journal of traditional, complementary, and alternative medicines 2012 Oct 1 ;10(1):15-20.

Protective role of {Artemisia afra} aqueous extract on tissue antioxidant defense systems in streptozotocin-induced diabetic rats

Abstract :

Changes in antioxidant capacity in the body as a result of oxidative stress play an important role in the development of diabetic complications. The aim of this study was to evaluate the effect of aqueous extract of Artemisia afra Jacq. ex Willd. on antioxidant defense systems in the liver and kidney of streptozotocin-induced diabetic rats. Administration of the extract to diabetic rats for 21 days significantly reduced blood glucose levels and increased body weight. The diabetic animals exhibited decreased levels of glutathione reductase (GR), glutathione peroxidase (GPx), superoxide dismutase (SOD) and reduced glutathione (GSH) in the liver and kidney, which were restored to near normal levels following treatment with the herb. The increased levels of lipid peroxidation observed in the tissues of diabetic rats were also reverted back to near normalcy after administering the extract. These findings revealed the protective role of A. afra on tissues by reducing oxidative stress which could be attributed to its flavonoids content. The efficacy of the plant compared favourably well with glibenclamide, a standard hypoglycemic drug.
Keywords : Artemisia afra ; antioxidant ; diabetes ; flavonoids ; free radicals ; oxidative stress

2013

Sunmonu TO1, Afolayan AJ.
Evaluation of Antidiabetic Activity and Associated Toxicity of Artemisia afra Aqueous Extract in Wistar Rats
Evidence-Based Complementary and Alternative Medicine. 2013 ;2013:929074. doi : 10.1155/2013/929074. Epub 2013 Jun 4.

Evaluation of Antidiabetic Activity and Associated Toxicity of Artemisia afra Aqueous Extract in Wistar Rats

Abstract :

Artemisia afra Jacq. ex Willd. is a widely used medicinal plant in South Africa for the treatment of diabetes. This study aimed to evaluate the hypoglycemic activity and possible toxicity effect of aqueous leaf extract of the herb administered at different dosages for 15 days in streptozotocin-induced diabetic rats. Administration of the extract at 50, 100, and 200 mg/kg body weight significantly (P < 0.05) increased body weight, decreased blood glucose levels, increased glucose tolerance, and improved imbalance in lipid metabolism in diabetic rats. These are indications of antidiabetic property of A. afra with 200 mg/kg body weight of the extract showing the best hypoglycemic action by comparing favourably well with glibenclamide, a standard hypoglycemic drug. The extract at all dosages tested also restored liver function indices and haematological parameters to normal control levels in the diabetic rats, whereas the kidney function indices were only normalized in the diabetic animals administered with 50 mg/kg body weight of the extract. This investigation clearly showed that in addition to its hypoglycemic activity, Artemisia afra may also protect the liver and blood against impairment due to diabetes. However, some kidney functions may be compromised at high dosages of the extract.

2015

Idris Ahmed Issa and Mohammed Hussen Bule
Hypoglycemic Effect of Aqueous and Methanolic Extract of Artemisia afra on Alloxan Induced Diabetic Swiss Albino Mice
Evidence-Based Complementary and Alternative Medicine Volume 2015, Article ID 752486, 5 pages

Hypoglycemic Effect of Aqueous and Methanolic Extract of Artemisia afra on Alloxan Induced Diabetic Swiss Albino Mice Evidence-Based

Open access article distributed under the Creative Commons Attribution License

Abstract

Diabetes mellitus is metabolic syndrome that causes disability, early death, and many other complications. Currently insulin and many synthetic drugs are used in diabetes treatment. However, these pharmaceutical drugs are too expensive particularly for sub-Saharan population in addition to their undesirable side effects. The present study was aimed to evaluate antidiabetic effect and toxicity level of Artemisia afra which was collected from its natural habitat in Bale Zone, around Goba town, 455 km southeast of Addis Ababa. Air dried aerial parts of Artemisia afra were separately extracted with both distilled water and 95% methanol.
Oral acute toxicity test was conducted on healthy Swiss albino mice. Antidiabetic effect of the aqueous and methanolic extracts of Artemisia afra was separately evaluated on alloxan induced diabetic mice at doses of 500, 750, and 1000mg/Kg body weight orally. The results indicate that mean lethal dose (LD50) for aqueous extract of Artemisia afra was 9833.4mg/Kg. Blood glucose level was significantly decreased by 24% (𝑝 < 0.005) and 56.9% (𝑝 < 0.0004) in groups that received aqueous extract of Artemisia afra at dose of 500mg/Kg and 750mg/Kg, respectively.The methanolic extract of Artemisia afra also significantly lowered blood glucose by 49.8% (𝑝 < 0.0001) at doses of 1000 mg/kg on the 5th hr. Aqueous extract of Artemisia afra was regarded as nontoxic and safe since its LD50 was found above 5000mg/Kg.Aqueous extract showed higher effect at relatively lower dose as compared to methanolic extract. The aqueous extract was screened positive for phytochemicals like flavonoids, polyphenols, and tannins that were reported to have antioxidant activity.

2016

Nikodimos Eshetu Mekbeb Afework, Eyasu Makonnen, Asfaw Debella, Wondwossen Ergete, Tesfaye Tolesss
Evaluation of the Acute and Sub-chronic Toxic Effects of Aqueous Leaf Extracts of Artemisia afra on Liver, Kidney and Some Blood Parameters in Wistar Rats
Advances in Bioscience and Bioengineering, Vol. 1, No. 1, 2016, pp. 1-9

Evaluation of the Acute and Sub-chronic Toxic Effects of Aqueous Leaf Extracts of {Artemisia afra} on Liver, Kidney and Some Blood Parameters in Wistar Rats

Abstract :

Background : Artemisia afra is a plant traditionally used for treatment of different diseases in many parts of the world including Ethiopia. Its effects on different organs, however, have not yet been investigated. The objective of the present study was, therefore, to evaluate the acute and sub-chronic toxic effects of aqueous leaf extracts of Artemisia afra on Liver, Kidney and some Blood parameters in Rats.

Methods : For acute toxicity study, aqueous extracts of the leaves were administered in a single dose of 200, 700, 1200, 2200, 3200, 4200 and 5000mg/kg body weight, while the low dose (600mg/kg) and triple of lower dose (1800mg/kg) were used for sub-chronic toxicity studies. Selected hematological and biochemical parameters of the blood followed by histopathological analysis were investigated after 90 days of daily administrations. The results were expressed as M ± SE, and differences at P < 0.05 were considered significant. Differences between the experimental and control groups were analyzed using one-way analysis of variance (ANOVA), followed by Dunnett’s T-test to determine their level of significance.

Results : The current study showed that the median oral lethal dose (LD50) was greater than 5000mg/kg. Acute toxicity study revealed some changes in general behavior of the rats above 3200mg/kg. The levels of blood parameters did not change though AST level decreased significantly in female animals after 90 days of sub-chronic treatment with 1800mg/kg. Histopathological presentations were generally normal though there were mild mononuclear leukocytic infiltrations around the central venules & portal areas of rats’ liver at both 600 and 1800mg/kg dose. Furthermore, minor tubulointerstitial leukocytic infiltrations were observed in small areas of kidney sections treated at higher dose.

Conclusion : The aqueous extract of Artemisia afra at the test doses did not show significant toxicity : the minor inflammatory changes observed in this study were not accompanied by significant change in any of the hematological and biochemical markers of liver injury. It might be a response to parenchymal cell death with causes ranging from infectious agents, exposure to toxicants, generation of toxic metabolites, and tissue anoxia. Keywords : Artemisia afra, Traditional Medicine, Toxicological Assessment

2017

Dabe, Nikodimos Eshetu, and Adane Teshome Kefale.
Antidiabetic Effects of Artemisia Species : A Systematic Review
Ancient Science of Life. 2017 Apr-Jun ;36(4):175-181

Evaluation of the Acute and Sub-chronic Toxic Effects of Aqueous Leaf Extracts of {Artemisia afra} on Liver, Kidney and Some Blood Parameters in Wistar Rats

Abstract :

Background : Over the last century, human life style and food habits have drastically changed which lead to various chronic diseases. Diabetes mellitus is one such disease which is causing serious problems to human health. Allopathic drugs are not much effective in handling the disease and its complications. Hence focus has been turned towards the traditional system of medicine.

Methods : Experimental studies conducted on species of Artemisia on diabetic animal models and human published since the year 2000 until April, 2017 were reviewed. Each article was critically appraised by two independent reviewers for their methodological quality using the JBIMAStARI tool.

Result : A total of 14 studies were included in this review and the blood glucose data obtained from these critically reviewed studies clearly showed that both the aqueous and alcoholic extracts of species of Artemisia produced significant hypoglycemic effects in alloxan, Streptozotocin and high fat diet induced diabetic animals and diabetic humans with different mechanisms of action as compared to standard antidiabetic medications.

Discussion and Conclusion : The antidiabetic effect of single or multiple doses of aqueous and alcoholic extracts of Artemisia species was due to the active compounds of these plants and they all are effective in lessening the blood glucose level in all of those experimental studies. Despite the presence of known antidiabetic medicines in the pharmaceutical market, therapeutic remedies from these medicinal plants have been utilized with success to treat this disorder and its complications with a relatively less side effects.

Mis en ligne par La vie re-belle
 3/04/2020
 https://www.lavierebelle.org/artemisia-afra-etudes-in-vivo-des

 Documents

 A pharmacological investigation of the hypoglycemic activity of Artemisia afra Jacq.
PDF Planta Medica
 Planta Medica
 Artemisia afra Jacq. ameliorates oxidative stress in the pancreas of streptozotocin-induced diabetic Wistar rats
PDF 
 Bioscience, Biotechnology, and Biochemistry
 Protective role of {Artemisia afra} aqueous extract on tissue antioxidant defense systems in streptozotocin-induced diabetic rats
PDF 
 African journal of traditional, complementary, and alternative medicines
 Evaluation of Antidiabetic Activity and Associated Toxicity of Artemisia afra Aqueous Extract in Wistar Rats
PDF 
 Evidence-Based Complementary and Alternative Medicine

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